Protocols

A detailed protocol is a key component of maintaining rigor and reproducibility and supporting open science practices. Protocols.io allows us to develop, edit, and share our protocols.

iPSC

• Preparation of Feeder-Free iPSC Culture: doi.org/10.17504/protocols.io.x83fryn
• iPSC Cell Culture – Maintenance and Expansion: doi.org/10.17504/protocols.io.9pwh5pe
• iPSC Freezing: doi.org/10.17504/protocols.io.x8zfrx6
• Characterization of iPSC: doi.org/10.17504/protocols.io.x82frye

Neural Induction for Neural Progenitors, Neurons, and Astrocytes

• Preparation of Tissue Culture Plates for Neural Rosettes and Neural Progenitors: doi.org/10.17504/protocols.io.x85fry6
• Neural Aggregate Formation: doi.org/10.17504/protocols.io.x84fryw
• Neural Rosette Formation and Selection: doi.org/10.17504/protocols.io.x86frze
• Neural Progenitor Expansion: doi.org/10.17504/protocols.io.x88frzw
• Neural Progenitor Banking: doi.org/10.17504/protocols.io.x89frz6
• Neural Rosette Banking: doi.org/10.17504/protocols.io.x87frzn
• Thawing Rosettes and NPC: doi.org/10.17504/protocols.io.x9afr2e
• Differentiation of NPC into Cortical Neurons: doi.org/10.17504/protocols.io.x9bfr2n
• iPSC Cortical Differentiation: doi.org/10.17504/protocols.io.x9cfr2w

Seamless Genome Editing of iPSC

• Genomic Editing of iPSC: doi.org/10.17504/protocols.io.yayfsfw
• Footprint-Free Genome Editing of iPSC Using Alt-R CRISPR/Cas9: doi.org/10.17504/protocols.io.bfmmjk46
• Nucleofection of iPSC: doi.org/10.17504/protocols.io.x62frge
• Serial Dilution of Nucleofected iPSC Pools: doi.org/10.17504/protocols.io.x76frre
• Picking Clones: doi.org/10.17504/protocols.io.x77frrn
• Splitting 96 Well Plates for gDNA Extraction and Continuing Culture: doi.org/10.17504/protocols.io.x78frrw
• Splitting 96 Well Plates for gDNA Extraction and Freezing Down: doi.org/10.17504/protocols.io.x79frr6
• Screening Edited iPSC Clones: doi.org/10.17504/protocols.io.yazfsf6
• iPSC gDNA Extraction for Screening Edited Clones: doi.org/10.17504/protocols.io.x8afrse
• iPSC PCR for Screening Edited Clones: doi.org/10.17504/protocols.io.x8bfrsn
• iPSC Restriction Digest for Screening Edited Clones: doi.org/10.17504/protocols.io.x8sfrwe
• Sanger Sequencing: doi.org/10.17504/protocols.io.x8tfrwn
• Thawing iPSC Plate: doi.org/10.17504/protocols.io.x8ufrww

Microglia

• Differentiation of iPSC into Microglia-Like Cells (iMGL):  doi.org/10.17504/protocols.io.be43jgyn

Fibroblasts

• Thawing, Expanding, and Freezing Human Fibroblasts: doi.org/10.17504/protocols.io.bgunjwve

Astrocytes

• An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells: https://pubmed.ncbi.nlm.nih.gov/28757165/

Cerebral Organoids

• For information on Cerebral Organoid culture, please refer to ELAVL4, splicing and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids: https://pubmed.ncbi.nlm.nih.gov/34314701/